investigation of mmp-2 and mmp-9 activities in canine sera with dilated cardiomyopathy

نویسندگان

s. chegeni

z. khaki

d. shirani

a. vajhi

m. taheri

چکیده

dilated cardiomyopathy (dcm) is accompanied by myocytes and connective tissue changes. matrix metalloproteinases (mmps) play important roles in cardiac remodeling. it seems that the gelatinases (mmp-2 and mmp-9) are effective enzymes in cardiomyopathy. dilated cardiomyopathy was confirmed in 22 dogs (patient group) including 11 female and 11 male by clinical examination, auscultation, thoracic radiography and echocardiography. 17 healthy dogs (control group) with similar weight and breedto patients were also selected from referred cases to small animal hospital of the veterinary faculty of tehran university and the same diagnostic procedures were performed on them. after that, serum mmp-2 and mmp-9 of control and patient groups were measured by semi-quantitative zymography. semiquantitative analysis of zymograms from canine serums with dcm showed that total mmp-9 in patients is more than control group, while there was no significant difference in total mmp-2 between the two groups. pro-mmp-2 was not detected in patient group but its active form was present in both groups, of course mmp-2 activity inpatients was significantly more than control. active form of mmp-9 was detected only in patients. although pro-mmp-9 was present in both groups, its level in control group was significantly higher than patients. the heart enlargement was observed in the left, right or both parts. statistically significant differences in active form of mmp-2 and mmp-9 levels were observed between different groups of heart enlargement (right, left and both parts) compared to control but this difference was not significant considering chambers affected and vhs (vertebral heart score) groups. in conclusion, although there are some changes in serum mmp-2 and mmp-9 levels in canine dcm, it seems that increase of mmp-9 is more prominent than mmp-2 and neither of them were affected by heart enlargement or vhs grade.

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Investigation of MMP-2 and MMP-9 activities in canine sera with dilated cardiomyopathy

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Investigation of MMP-2 and MMP-9 activities in canine sera with dilated cardiomyopathy.

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عنوان ژورنال:
iranian journal of veterinary research

ناشر: shiraz university

ISSN 1728-1997

دوره 16

شماره 2 2015

کلمات کلیدی
[ ' d c m ' , ' m a t r i x m e t a l l o p r o t e i n a s e ' , ' m m p ' , 2 , ' m m p ' , 9 , ' z y m o g r a p h y ' ]

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